By Daniel Klionsky
Quite in instances of rigidity, like hunger and disorder, better organisms have an inner mechanism of their cells for chewing up and recycling components of themselves. the method of inner "house-cleaning" within the telephone is named autophagy - actually self-eating. Breakthroughs in realizing the molecular foundation of autophagy got here after the cloning of ATG1 in yeast. those ATG genes in yeast have been the stepping stones to the explosion of study into the molecular research of autophagy in larger eukaryotes. sooner or later, this learn might help to layout scientific ways which could activate autophagy and halt tumor development. during this quantity, a powerful slate of tools for engaging in study are provided, constructing a consensus of applicable standards for tracking autophagy. besides with Autophagy: decrease Eukaryotes, this quantity marks the seminal choice of equipment within the burgeoning box of autophagy.
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Additional info for Autophagy in mammalian systems
And Hanada, K. (2008). Consideration about negative controls for LC3 and expression vectors for four colored fluorescent protein-LC3 negative controls. Autophagy 4, 131–134. Tanida, I. , and Kominami, E. (2005). Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1, 84–91. , and Kominami, E. (2008). LC3 and Autophagy. Methods Mol. Biol. 445, 77–88. C H A P T E R T W O Methods for Monitoring Autophagy Using GFP-LC3 Transgenic Mice Noboru Mizushima* Contents 1.
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